A general biochemical kinetics data fitting algorithm for quasi-steady-state detection
Articles
Justinas V. Daugmaudis
Vilnius University
Audrius Laurynėnas
Vilnius University
Juozas Kulys
Vilnius University
Feliksas Ivanauskas
Vilnius University
Published 2016-12-15
https://doi.org/10.15388/LMR.A.2016.03
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Keywords

biochemical kinetics
differential equations
curve fitting
reaction rate constants
phosphatase
4-nitrophenylphosphate

How to Cite

Daugmaudis, J.V. (2016) “A general biochemical kinetics data fitting algorithm for quasi-steady-state detection”, Lietuvos matematikos rinkinys, 57(A), pp. 12–17. doi:10.15388/LMR.A.2016.03.

Abstract

We develop a general algorithm for fitting the biochemical kinetics data. The developed algorithm searches and analyzes numerous minima. This approach allows us to analyze biochemical data without a priori quasi-steady-state assumptions. The algorithm allows us to treat the biochemical kinetics data that has varying degree of steadiness. We test the approach by analyzing experiment data from 4-nitrophenyl phosphate hydrolysis with alkaline phosphatase.

 

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