Abstract
Background. The diagnostic efficacy of the molecular analysis that included determination of papillary thyroid carcinoma (PTC) marker gene expression levels and BRAF mutation in fine-needle aspiration biopsy material was evaluated in a prospective study of patients with thyroid nodules.
Materials and methods. Totally, 36 patients (29 females and 7 males) with thyroid nodules were included in the study. The mRNA expression of genes (SFTPB and TFF3) was estimated in relation to a housekeeping gene level (KPNA4) by means of duplex RT-PCR followed by the band intensity measurement. Detection of BRAF mutation was performed by PCR followed by direct sequencing.
Results. In 25/32 (78.1%) cases, results of the molecular test were in agreement with the cytological diagnosis (7/7 PTC and 18/25 non-PTC) further confirmed by histological examination of tissues surgically removed from all seven PTC patients and 10 individuals with benign nodules. In 7/32 patients (21.9%) there was a discrepancy between cytological findings and molecular results, which revealed a benign nodule and a PTC-like pattern, respectively. Upon a repeated examination of five of these patients about one year later, three were cytologically diagnosed with PTC (all patients had been operated on), and the diagnosis of the other two patients remained unchanged.
Conclusions. The results have demonstrated that the molecular analysis of FNAB material is an informative means of the preoperative diagnosis of thyroid nodules as it allows identification of patients with suspected PTC before other diagnostically significant changes take place.
Keywords: papillary thyroid carcinoma, fine-needle aspiration biopsy, preoperative diagnosis, molecular test
Downloads
Download data is not yet available.
Most read articles by the same author(s)
-
Mindaugas Gailiušas,
Judita Andrejaitienė,
Edmundas Širvinskas,
Darijus Krasauskas,
Milda Švagždienė,
Birutė Kumpaitienė,
Association between serum biomarkers and postoperative delirium after cardiac surgery
,
Acta medica Lituanica: Vol. 26 No. 1 (2019): Acta Medica Lituanica
-
Ieva Norkienė,
Raquel d’Espiney,
Juan F. Martin-Lazaro,
Effectiveness of high-flow nasal oxygen therapy in management of acute hypoxemic and hypercapnic respiratory failure
,
Acta medica Lituanica: Vol. 26 No. 1 (2019): Acta Medica Lituanica
-
Audrius Aurelijus Pilypas,
Giedrutė Raišelienė,
Jurgita Valaikienė,
Lyme disease and heart transplantation: presentation of a clinical case and a literature review
,
Acta medica Lituanica: Vol. 26 No. 3 (2019): Acta Medica Lituanica
-
Monika Kapitančukė,
Agnija Vaščiūnaitė,
Rasa Augustinienė,
Jūratė Sakalinskienė,
Gražina Kleinotienė,
Primary gastric Burkitt lymphoma-induced anaemia: a case report and a literature review
,
Acta medica Lituanica: Vol. 25 No. 1 (2018)
-
Audrius Untanas,
Mantas Trakymas,
Indrė Lekienė,
Rūta Briedienė,
Von Hippel-Lindau syndrome and renal tumours: radiological diagnostic and treatment options. A case report and literature review
,
Acta medica Lituanica: Vol. 27 No. 1 (2020): Acta medica Lituanica
-
Emrah Doğan,
Sabri Serhan Olcay,
Tuba Çınar Olcay,
Utku Tapan,
Ozge Oral Tapan,
Fatih Alaşan,
A case of post-COVID-19 fibrosis mimicking Thoracic Manifestation of Ankylosing Spondylitis.
,
Acta medica Lituanica: Vol. 29 No. 1 (2022): Acta medica Lituanica
-
Aurelija Kemežytė,
Dario Piombino-Mascali,
Bioarchaeological Notes on the Commingled Human Remains Held in the Church of Saint Francis of Paola, Sant’Angelo di Brolo, Sicily, Italy
,
Acta medica Lituanica: Vol. 29 No. 1 (2022): Acta medica Lituanica
-
Tara Prasad Tripathy,
Yashwant Patidar,
Karamvir Chandel,
Annapoorani Varadarajan,
Vikrant Sood,
Shalini Thapar Laroia,
Embryonal Rhabdomyosarcoma of the Biliary Tree as a Differential in a Paediatric Patient Presenting with Biliary Dilatation: Not Always a Choledochal Cyst
,
Acta medica Lituanica: Vol. 29 No. 1 (2022): Acta medica Lituanica
-
Vasiliki Epameinondas Georgakopoulou,
Georgios Petsinis,
Konstantinos Mantzouranis,
Christos Damaskos,
Despoina Melemeni,
Aikaterini Gkoufa,
Serafeim Chlapoutakis,
Nikolaos Garmpis,
Pagona Sklapani,
Nikolaos Trakas,
Xanthi Tsiafaki,
First Detection of Human Coronavirus HKU1 in Greece, in an Immunocompromised Patient With Severe Lower Respiratory Tract Infection
,
Acta medica Lituanica: Vol. 28 No. 1 (2021): Acta medica Lituanica
-
Pranjal Phukan,
Kalyan Sarma,
Donboklang Lynser,
Barun Kumar Sharma,
Deb Kumar Baruah,
Bishwajit Saikia,
Binoy Kumar Singh,
Endovascular Parent Artery Occlusion for Intracranial Aneurysms is a Viable, Cost-Effective Alternative: An Institutional Experience from Northeast India
,
Acta medica Lituanica: Vol. 28 No. 2 (2021): Acta medica Lituanica