Abstract
Background. The diagnostic efficacy of the molecular analysis that included determination of papillary thyroid carcinoma (PTC) marker gene expression levels and BRAF mutation in fine-needle aspiration biopsy material was evaluated in a prospective study of patients with thyroid nodules.
Materials and methods. Totally, 36 patients (29 females and 7 males) with thyroid nodules were included in the study. The mRNA expression of genes (SFTPB and TFF3) was estimated in relation to a housekeeping gene level (KPNA4) by means of duplex RT-PCR followed by the band intensity measurement. Detection of BRAF mutation was performed by PCR followed by direct sequencing.
Results. In 25/32 (78.1%) cases, results of the molecular test were in agreement with the cytological diagnosis (7/7 PTC and 18/25 non-PTC) further confirmed by histological examination of tissues surgically removed from all seven PTC patients and 10 individuals with benign nodules. In 7/32 patients (21.9%) there was a discrepancy between cytological findings and molecular results, which revealed a benign nodule and a PTC-like pattern, respectively. Upon a repeated examination of five of these patients about one year later, three were cytologically diagnosed with PTC (all patients had been operated on), and the diagnosis of the other two patients remained unchanged.
Conclusions. The results have demonstrated that the molecular analysis of FNAB material is an informative means of the preoperative diagnosis of thyroid nodules as it allows identification of patients with suspected PTC before other diagnostically significant changes take place.
Keywords: papillary thyroid carcinoma, fine-needle aspiration biopsy, preoperative diagnosis, molecular test
Downloads
Download data is not yet available.
Most read articles by the same author(s)
-
Albertas Ulys,
Agnė Ulytė,
Pavel Dziameshka,
Oleg Sukonko,
Sergei Krasny,
Sergei Polyakov,
Giedrė Smailytė,
High-risk prostate cancer: factors predicting biochemical recurrence after radical prostatectomy
,
Acta medica Lituanica: Vol. 22 No. 3 (2015)
-
Rimantas Jankauskas,
Editorial Board and Table of Contents
,
Acta medica Lituanica: Vol. 27 No. 2 (2020): Acta medica Lituanica
-
Narimantas Evaldas SAMALAVIČIUS,
Alfredas KILIUS,
Kęstutis PETRULIS,
Simona LETAUTIENĖ,
Rūta GRIGIENĖ,
Rūta BRIEDIENĖ,
Edita MIŠEIKYTĖ-KAUBRIENĖ,
Transanal endoscopic microsurgery: results of the first 50 cases
,
Acta medica Lituanica: Vol. 19 No. 1 (2012)
-
Živilė Gudlevičienė,
Genovefa Chvatovič,
New challenges in the biomedical science: biobanking problems and solutions
,
Acta medica Lituanica: Vol. 20 No. 4 (2013)
-
Justinas KRETAVIČIUS,
Rūta DUBAKIENĖ,
Jonas BALŽEKAS,
Viktoras ŠAFERIS,
Rape and buckwheat honey allergy in relation to heat treatment
,
Acta medica Lituanica: Vol. 17 No. 3-4 (2010)
-
Laimutė RUTKOVSKIENĖ,
Laima PLĖŠNIENĖ,
Daiva SENDIULIENĖ,
Alfonsas STANČIUS,
Jurgita LIUTKEVIČIŪTĖ-NAVICKIENĖ,
Sensitization of rat C6 glioma cells to ionizing radiation by porphyrins
,
Acta medica Lituanica: Vol. 18 No. 2 (2011)
-
Andrew BUSH,
Problematic, severe asthma in children: a new concept and how to manage it
,
Acta medica Lituanica: Vol. 17 No. 1-2 (2010)
-
Yemisi Olukemi Adesiji,
Racheal Adekemi Akanni,
Olusegun Adelowo Adefioye,
Sunday Samuel Taiwo,
In vitro antimicrobial activity of some plant extracts against Arcobacter butzleri and Arcobacter cryaerophilus
,
Acta medica Lituanica: Vol. 19 No. 1 (2012)
-
Augustina Jankauskienė,
Daiva Vaičiūnienė,
Indrė Stacevičienė,
Prevalence of lower urinary tract symptoms in Vilnius area children and adolescents
,
Acta medica Lituanica: Vol. 21 No. 1 (2014)
-
Arida Buivydienė,
Viktorija Basytė,
Laura Mašalaitė,
Danguolė Stanislovaitytė,
Jurgita Adomavičiūtė,
Jonas Valantinas,
The impact of hepatitis C virus infection on early post-liver transplantation complications and early graft function in a single low-volume liver transplant center
,
Acta medica Lituanica: Vol. 22 No. 1 (2015)