Abstract
Background. The diagnostic efficacy of the molecular analysis that included determination of papillary thyroid carcinoma (PTC) marker gene expression levels and BRAF mutation in fine-needle aspiration biopsy material was evaluated in a prospective study of patients with thyroid nodules.
Materials and methods. Totally, 36 patients (29 females and 7 males) with thyroid nodules were included in the study. The mRNA expression of genes (SFTPB and TFF3) was estimated in relation to a housekeeping gene level (KPNA4) by means of duplex RT-PCR followed by the band intensity measurement. Detection of BRAF mutation was performed by PCR followed by direct sequencing.
Results. In 25/32 (78.1%) cases, results of the molecular test were in agreement with the cytological diagnosis (7/7 PTC and 18/25 non-PTC) further confirmed by histological examination of tissues surgically removed from all seven PTC patients and 10 individuals with benign nodules. In 7/32 patients (21.9%) there was a discrepancy between cytological findings and molecular results, which revealed a benign nodule and a PTC-like pattern, respectively. Upon a repeated examination of five of these patients about one year later, three were cytologically diagnosed with PTC (all patients had been operated on), and the diagnosis of the other two patients remained unchanged.
Conclusions. The results have demonstrated that the molecular analysis of FNAB material is an informative means of the preoperative diagnosis of thyroid nodules as it allows identification of patients with suspected PTC before other diagnostically significant changes take place.
Keywords: papillary thyroid carcinoma, fine-needle aspiration biopsy, preoperative diagnosis, molecular test
Downloads
Download data is not yet available.
Most read articles by the same author(s)
-
Laima BLOZNELYTĖ-PLĖŠNIENĖ,
Aleksandras MORDAS,
Vytautas ČEPULIS,
Jonas VENIUS,
Jurgita LIUTKEVIČIŪTĖ-NAVICKIENĖ,
Laimutė RUTKOVSKIENĖ,
Valerijus OSTAPENKO,
Konstantinas Povilas VALUCKAS,
Fluorescence diagnostics of oral cancer
,
Acta medica Lituanica: Vol. 18 No. 2 (2011)
-
Anna Kornete,
Diana Bokucava,
Natalija Vedmedovska,
Fetal Sacrococcygeal Teratoma: A Case Report of a Giant Tumor with an Excellent Outcome
,
Acta medica Lituanica: Vol. 30 No. 2 (2023): Acta medica Lituanica
-
Monique Boukobza,
Jurgita Ušinskienė,
Simona Letautienė,
Case report of ervical myelopathy due to ossification of the posterior longitudinal ligament in association with flavum ligament ossification: MRI and CT appearance
,
Acta medica Lituanica: Vol. 20 No. 2 (2013)
-
Ieva Norkienė,
Ieva Kažukauskienė,
Robertas Samalavičius,
Kęstutis Ručinskas,
Successful application of thrombolysis and angioplasty in case of mechanical aortic valve and coronary graft thrombosis
,
Acta medica Lituanica: Vol. 23 No. 1 (2016)
-
Ramunė Mineikytė,
Ernestas Janulionis,
Vydmantas Atkočius,
Laimonas Jaruševičius,
Aista Plieskienė,
Jonas Gečas,
The changes of radiotherapy in Lithuania: infrastructure, utilization rate, and cost
,
Acta medica Lituanica: Vol. 23 No. 1 (2016)
-
Ieva Vincerževskienė,
Agnė Krilavičiūtė,
Giedrė Smailytė,
Trends in cancer incidence in Lithuania between 1991 and 2010
,
Acta medica Lituanica: Vol. 20 No. 4 (2013)
-
Žymantas Jagelavičius,
Vytautas Jovaišas,
Mindaugas Matačiūnas,
Narimantas Evaldas Samalavičius,
Ričardas Janilionis,
Video-assisted thoracic surgery in pleural empyema: predictors of complications and treatment failures
,
Acta medica Lituanica: Vol. 22 No. 2 (2015)
-
Birutė SURINĖNAITĖ,
Gintautas DOMŽA,
Janina DIDŽIAPETRIENĖ,
Gražina PRASMICKIENĖ,
Audronė JAKAITIENĖ,
The status of antioxidative system in pregnant women infected with human papilloma virus
,
Acta medica Lituanica: Vol. 17 No. 3-4 (2010)
-
Deb K. Boruah,
Daisy Vishwakarma,
Pallavi Gogoi,
Navneet R Lal ,
Arup Deuri,
Utility of High-Resolution Ultrasonography in the Evaluation of Posterior Segment Ocular Lesions Using Sensitivity and Specificity
,
Acta medica Lituanica: Vol. 30 No. 2 (2023): Acta medica Lituanica
-
Jolanta DADONIENĖ,
Dalius JATUŽIS,
Arvydas LAURINAVIČIUS,
The diagnostic value of ultrasound examination in temporal arteritis
,
Acta medica Lituanica: Vol. 17 No. 1-2 (2010)