Abstract
Background. The diagnostic efficacy of the molecular analysis that included determination of papillary thyroid carcinoma (PTC) marker gene expression levels and BRAF mutation in fine-needle aspiration biopsy material was evaluated in a prospective study of patients with thyroid nodules.
Materials and methods. Totally, 36 patients (29 females and 7 males) with thyroid nodules were included in the study. The mRNA expression of genes (SFTPB and TFF3) was estimated in relation to a housekeeping gene level (KPNA4) by means of duplex RT-PCR followed by the band intensity measurement. Detection of BRAF mutation was performed by PCR followed by direct sequencing.
Results. In 25/32 (78.1%) cases, results of the molecular test were in agreement with the cytological diagnosis (7/7 PTC and 18/25 non-PTC) further confirmed by histological examination of tissues surgically removed from all seven PTC patients and 10 individuals with benign nodules. In 7/32 patients (21.9%) there was a discrepancy between cytological findings and molecular results, which revealed a benign nodule and a PTC-like pattern, respectively. Upon a repeated examination of five of these patients about one year later, three were cytologically diagnosed with PTC (all patients had been operated on), and the diagnosis of the other two patients remained unchanged.
Conclusions. The results have demonstrated that the molecular analysis of FNAB material is an informative means of the preoperative diagnosis of thyroid nodules as it allows identification of patients with suspected PTC before other diagnostically significant changes take place.
Keywords: papillary thyroid carcinoma, fine-needle aspiration biopsy, preoperative diagnosis, molecular test
Downloads
Download data is not yet available.
Most read articles by the same author(s)
-
Petros Galanis,
Irene Vraka,
Aglaia Katsiroumpa,
Olga Siskou,
Olympia Konstantakopoulou,
Theodoros Katsoulas,
Theodoros Mariolis-Sapsakos,
Daphne Kaitelidou,
Attitudes toward COVID-19 Pandemic among Fully Vaccinated Individuals: Evidence from Greece Two Years after the Pandemic
,
Acta medica Lituanica: Vol. 29 No. 2 (2022): Acta medica Lituanica
-
Christos Damaskos,
Nikolaos Garmpis,
Anna Garmpi,
Vasiliki Epameinondas Georgakopoulou,
Alexandros Patsouras,
Georgia Sypsa,
Athanasios Syllaios,
Efstathios A. Antoniou,
Ethical Dilemma: Is it Worthwhile Operating an End-Stage Pancreatic Cancer Patient with Acute Mesenteric Artery Ischemia?
,
Acta medica Lituanica: Vol. 28 No. 2 (2021): Acta medica Lituanica
-
Jurijus Makevičius,
Beata Kirstukaitė,
Renata Komiagienė,
Arūnas Želvys,
Feliksas Jankevičius,
Marius Miglinas,
Risk Factors of Chronic Kidney Disease after Partial Nephrectomy
,
Acta medica Lituanica: Vol. 29 No. 2 (2022): Acta medica Lituanica
-
Edita Baltruškevičienė,
Ugnius Mickys,
Tadas Žvirblis,
Rokas Stulpinas,
Teresė Pipirienė Želvienė,
Eduardas Aleknavičius,
Significance of KRAS, NRAS, BRAF and PIK3CA mutations in metastatic colorectal cancer patients receiving Bevacizumab: a single institution experience
,
Acta medica Lituanica: Vol. 23 No. 1 (2016)
-
Šarūnas Judickas,
Dalia Gineitytė,
Greta Kezytė,
Ernestas Gaižauskas,
Mindaugas Šerpytis,
Jūratė Šipylaitė,
Is the Trendelenburg position the only way to better visualize internal jugular veins?
,
Acta medica Lituanica: Vol. 25 No. 3 (2018)
-
Karolis Baronas,
Tautvydas Rančelis,
Aidas Pranculis,
Ingrida Domarkienė,
Laima Ambrozaitytė,
Vaidutis Kučinskas,
Novel human genome variants associated with alcohol use disorders identified in a Lithuanian cohort
,
Acta medica Lituanica: Vol. 25 No. 1 (2018)
-
Deb K. Boruah,
Bidyut Bikash Gogoi,
Kuntal Kanti Das,
Kalyan Sarma,
Pranjal Phukan,
Binoy Kumar Singh,
Karuna Hazarika,
Awadhesh Jaiswal,
Added value of 3D-DRIVE and SWI Magnetic Resonance Imaging Sequences in Intraventricular Neurocysticercosis (IVNCC): An Institutional Experience from Northeast India
,
Acta medica Lituanica: Vol. 28 No. 2 (2021): Acta medica Lituanica
-
Jihad El Anzaoui,
COMMENTARY ON : " A Calculus “Toy” in the Bladder. A Case Report of Rare Entity and Comprehensive Review of the Literature"
,
Acta medica Lituanica: Vol. 29 No. 1 (2022): Acta medica Lituanica
-
Dibakar Borthakur,
Rajesh Kumar,
Vidhu Dhawan,
Rima Dada,
Bilateral Renal Hilar Nutcracker Phenomenon in a Male Cadaver
,
Acta medica Lituanica: Vol. 31 No. 1 (2024): Acta medica Lituanica
-
Vilma Kuzminskaitė,
Ieva Slauzgalvytė,
Greta Bukelytė,
Greta Bruzytė,
Eglė Kontrimavičiūtė,
Effects of sevoflurane and desflurane on early cognitive changes after thyroid surgery: interim results
,
Acta medica Lituanica: Vol. 26 No. 1 (2019): Acta Medica Lituanica