Abstract
Background. The diagnostic efficacy of the molecular analysis that included determination of papillary thyroid carcinoma (PTC) marker gene expression levels and BRAF mutation in fine-needle aspiration biopsy material was evaluated in a prospective study of patients with thyroid nodules.
Materials and methods. Totally, 36 patients (29 females and 7 males) with thyroid nodules were included in the study. The mRNA expression of genes (SFTPB and TFF3) was estimated in relation to a housekeeping gene level (KPNA4) by means of duplex RT-PCR followed by the band intensity measurement. Detection of BRAF mutation was performed by PCR followed by direct sequencing.
Results. In 25/32 (78.1%) cases, results of the molecular test were in agreement with the cytological diagnosis (7/7 PTC and 18/25 non-PTC) further confirmed by histological examination of tissues surgically removed from all seven PTC patients and 10 individuals with benign nodules. In 7/32 patients (21.9%) there was a discrepancy between cytological findings and molecular results, which revealed a benign nodule and a PTC-like pattern, respectively. Upon a repeated examination of five of these patients about one year later, three were cytologically diagnosed with PTC (all patients had been operated on), and the diagnosis of the other two patients remained unchanged.
Conclusions. The results have demonstrated that the molecular analysis of FNAB material is an informative means of the preoperative diagnosis of thyroid nodules as it allows identification of patients with suspected PTC before other diagnostically significant changes take place.
Keywords: papillary thyroid carcinoma, fine-needle aspiration biopsy, preoperative diagnosis, molecular test
Downloads
Download data is not yet available.
Most read articles by the same author(s)
-
Meda Sutkevičiūtė,
Monika Stančiukaitė,
Giedrė Bulotienė,
Individual Meaning-Centered Psychotherapy for palliative cancer patients in Lithuania. A case report
,
Acta medica Lituanica: Vol. 24 No. 1 (2017)
-
Veena Mariam Joseph,
Donboklang Lynser,
Iadarilang Tiewsoh,
Kaustuv Dutta,
Pranjal Phukan,
Chhunthang Daniala,
Tracheal Diverticulum in SARS-CoV-2 Patients on Non-Invasive Ventilation A not so “Spontaneous” Cause of Pneumomediastinum? An imaging Pictorial Presentation of Two Cases with Review of Literature
,
Acta medica Lituanica: Vol. 28 No. 2 (2021): Acta medica Lituanica
-
Arun Kumar Gunasekaran,
Amit Malviya,
Tony Ete,
Animesh Mishra,
Bhupen Barman,
Md Jamil,
Donboklang Lynser,
Hepatocellular Carcinoma with Inferior Vena Cava and Right Atrium Tumor Thrombus
,
Acta medica Lituanica: Vol. 28 No. 2 (2021): Acta medica Lituanica
-
Kristina Norvilaitė,
Aurelija Peštenytė,
Diana Bužinskienė,
Gražina Drąsutienė,
Audronė Arlauskienė,
Tomas Poškus,
Andrej Ostapenko,
Vasa praevia: a case report and literature review
,
Acta medica Lituanica: Vol. 23 No. 1 (2016)
-
Robert G.Hahn,
Clinical pharmacology of infusion fluids
,
Acta medica Lituanica: Vol. 19 No. 3 (2012)
-
Audrius Dulskas,
Žygimantas Kuliešius,
Narimantas E. Samalavičius,
Laparoscopic colorectal surgery for colorectal polyps: experience of ten years
,
Acta medica Lituanica: Vol. 24 No. 1 (2017)
-
Aikaterini Selntigia,
Benedetto Neri,
Consuelo Russo,
Francesco Giuseppe Martire,
Giorgia Soreca,
Dario Biasutto,
Francesco Maria Di Matteo,
Caterina Exacoustos,
A Rare Sigmoid-Colon Schwannoma in a Premenopausal Woman: A Case Report
,
Acta medica Lituanica: Vol. 30 No. 2 (2023): Acta medica Lituanica
-
Valentina GINEVIČIENĖ,
Erinija PRANCKEVIČIENĖ,
Kazys MILAŠIUS,
Vaidutis KUČINSKAS,
Relating fitness phenotypes to genotypes in Lithuanian elite athletes
,
Acta medica Lituanica: Vol. 17 No. 1-2 (2010)
-
Jolita Račkauskienė,
Vaida Gedvilaitė,
Mindaugas Matačiūnas,
Mažvilė Abrutytė,
Edvardas Danila,
Prognostic value of Mastora obstruction score in acute pulmonary embolism
,
Acta medica Lituanica: Vol. 26 No. 4 (2019): Acta Medica Lituanica
-
Jelena Rascon,
Giedrė Smailytė,
Improvement in childhood cancer survival in Lithuania over three decades
,
Acta medica Lituanica: Vol. 27 No. 1 (2020): Acta medica Lituanica